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Scanning Electron Microscopy (SEM) images showing the morphological effects of silver nanoparticles synthesized from C. arvensis. ( A – C ) demonstrate the effect on Gram-positive S. aureus (MRSA and ST), E-H demonstrate the effect <t>on</t> <t>Gram-negative</t> E. coli ESBL and ST) bacterial strains. described above, and the cells exhibited a division-related septum, broadening, and cytoplasm leakage. Scanning electron microscope images showed that the untreated control groups ( A , C , E , G ), bacterial cells exhibited normal morphology with intact cell walls and smooth surfaces. In contrast, treated cells ( B , D , F , H ) showed significant morphological changes: S. aureus cells displayed shrinkage and deformations in their spherical shape, while E. coli cells exhibited marked structural disintegration and severe membrane damage. These observations confirm the destructive effects of silver nanoparticles on bacterial cell integrity. Most of the cells were damaged and perforated, which changed the cell structure and shape, and it was noted that some of them were empty. In addition, most cells appeared to stick together compared to untreated cells. As is clear in .
Standard Gram Negative Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Scanning Electron Microscopy (SEM) images showing the morphological effects of silver nanoparticles synthesized from C. arvensis. ( A – C ) demonstrate the effect on Gram-positive S. aureus (MRSA and ST), E-H demonstrate the effect <t>on</t> <t>Gram-negative</t> E. coli ESBL and ST) bacterial strains. described above, and the cells exhibited a division-related septum, broadening, and cytoplasm leakage. Scanning electron microscope images showed that the untreated control groups ( A , C , E , G ), bacterial cells exhibited normal morphology with intact cell walls and smooth surfaces. In contrast, treated cells ( B , D , F , H ) showed significant morphological changes: S. aureus cells displayed shrinkage and deformations in their spherical shape, while E. coli cells exhibited marked structural disintegration and severe membrane damage. These observations confirm the destructive effects of silver nanoparticles on bacterial cell integrity. Most of the cells were damaged and perforated, which changed the cell structure and shape, and it was noted that some of them were empty. In addition, most cells appeared to stick together compared to untreated cells. As is clear in .
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Antimicrobial efficacy and cell‐damaging evaluation of selected seven peptide candidates. Experiments include: 1) Minimum Inhibitory Concentration (MIC) against seven Gram‐positive and <t>four</t> <t>Gram‐negative</t> bacterial strains. All peptides demonstrated potent antimicrobial activity and several exhibited broad‐spectrum activity against multiple bacteria strains. 2) Hemolytic activity measured using rat red blood cells, with EC 50 and MHC values exceeding 128 µg·mL −1 . 3) Cytotoxicity analysis using HEK293T cells. All peptides exhibited low toxicity with a CC 50 exceeding 128 µg·mL −1 .
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Scanning Electron Microscopy (SEM) images showing the morphological effects of silver nanoparticles synthesized from C. arvensis. ( A – C ) demonstrate the effect on Gram-positive S. aureus (MRSA and ST), E-H demonstrate the effect on Gram-negative E. coli ESBL and ST) bacterial strains. described above, and the cells exhibited a division-related septum, broadening, and cytoplasm leakage. Scanning electron microscope images showed that the untreated control groups ( A , C , E , G ), bacterial cells exhibited normal morphology with intact cell walls and smooth surfaces. In contrast, treated cells ( B , D , F , H ) showed significant morphological changes: S. aureus cells displayed shrinkage and deformations in their spherical shape, while E. coli cells exhibited marked structural disintegration and severe membrane damage. These observations confirm the destructive effects of silver nanoparticles on bacterial cell integrity. Most of the cells were damaged and perforated, which changed the cell structure and shape, and it was noted that some of them were empty. In addition, most cells appeared to stick together compared to untreated cells. As is clear in .

Journal: International Journal of Molecular Sciences

Article Title: Green Synthesis of Silver Nanoparticles with Antibacterial, Anti-Inflammatory, and Antioxidant Activity Using Convolvulus arvensis

doi: 10.3390/ijms27031210

Figure Lengend Snippet: Scanning Electron Microscopy (SEM) images showing the morphological effects of silver nanoparticles synthesized from C. arvensis. ( A – C ) demonstrate the effect on Gram-positive S. aureus (MRSA and ST), E-H demonstrate the effect on Gram-negative E. coli ESBL and ST) bacterial strains. described above, and the cells exhibited a division-related septum, broadening, and cytoplasm leakage. Scanning electron microscope images showed that the untreated control groups ( A , C , E , G ), bacterial cells exhibited normal morphology with intact cell walls and smooth surfaces. In contrast, treated cells ( B , D , F , H ) showed significant morphological changes: S. aureus cells displayed shrinkage and deformations in their spherical shape, while E. coli cells exhibited marked structural disintegration and severe membrane damage. These observations confirm the destructive effects of silver nanoparticles on bacterial cell integrity. Most of the cells were damaged and perforated, which changed the cell structure and shape, and it was noted that some of them were empty. In addition, most cells appeared to stick together compared to untreated cells. As is clear in .

Article Snippet: Four bacterial strains were used in this study: one standard Gram-positive strain ( Staphylococcus aureus ATCC 29213 ), one standard Gram-negative strain ( Escherichia coli ATCC 25922 ), and two clinical isolates (SP1 and SP2) obtained from patient samples.

Techniques: Electron Microscopy, Synthesized, Microscopy, Control, Membrane

Antimicrobial efficacy and cell‐damaging evaluation of selected seven peptide candidates. Experiments include: 1) Minimum Inhibitory Concentration (MIC) against seven Gram‐positive and four Gram‐negative bacterial strains. All peptides demonstrated potent antimicrobial activity and several exhibited broad‐spectrum activity against multiple bacteria strains. 2) Hemolytic activity measured using rat red blood cells, with EC 50 and MHC values exceeding 128 µg·mL −1 . 3) Cytotoxicity analysis using HEK293T cells. All peptides exhibited low toxicity with a CC 50 exceeding 128 µg·mL −1 .

Journal: Advanced Science

Article Title: Mechanism‐Driven Screening of Membrane‐Targeting and Pore‐Forming Antimicrobial Peptides

doi: 10.1002/advs.202516470

Figure Lengend Snippet: Antimicrobial efficacy and cell‐damaging evaluation of selected seven peptide candidates. Experiments include: 1) Minimum Inhibitory Concentration (MIC) against seven Gram‐positive and four Gram‐negative bacterial strains. All peptides demonstrated potent antimicrobial activity and several exhibited broad‐spectrum activity against multiple bacteria strains. 2) Hemolytic activity measured using rat red blood cells, with EC 50 and MHC values exceeding 128 µg·mL −1 . 3) Cytotoxicity analysis using HEK293T cells. All peptides exhibited low toxicity with a CC 50 exceeding 128 µg·mL −1 .

Article Snippet: Bacterial strains included four Gram‐negative strains ( Acinetobacter baumanii ATCC 17978, Acinetobacter baumanii ATCC 19606, Escherichia coli ATCC 25922, Escherichia coli DH5 α ) and seven Gram‐positive strains ( Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212, Micrococcus luteus CMCC(B) 28001, Staphylococcus capitis ATCC 27842, Staphylococcus hominis ATCC 27844).

Techniques: Concentration Assay, Activity Assay, Bacteria